rabbit anti gabaar a4  (Alomone Labs)

Journal: International Journal of Molecular Sciences

Article Title: Altered GABA A Receptor Expression in the Primary Somatosensory Cortex of a Mouse Model of Genetic Absence Epilepsy

doi: 10.3390/ijms232415685

Figure Lengend Snippet: WB analysis for GABA A R β2, β3, and γ2 subunits in the primary SoCx. The data compare expression between control (NE) littermates and epileptic (E) stargazers. ( A – C ) Representative blots display tissue expression of GABA A R β2, β3, and γ2 subunits in the primary SoCx with β-actin as the loading control. Bar graphs represent the relative expression levels of GABA A R β2, β3, and γ2 subunits, respectively. Bar graphs show a lack of statistically significant change in whole-tissue primary SoCx expression levels for β2 (NE: 0.917 ± 0.091 [ n = 12], E: 0.678 ± 0.050 [ n = 10]; p = 0.093), β3 (NE: 1.000 ± 0.067 [ n = 12], E: 1.070 ± 0.109 [ n = 10]; p = 0.859), and γ2 (NE: 1.000 ± 0.159 [ n = 9], E: 0.946 ± 0.079 [ n = 7]; p = 0.900). The significance threshold was set at 0.05. ‘ns’ indicates no significant change found from analyses.

Article Snippet: Membranes were probed using GABA A R α1 (1:500; AGA-001, Alomone, Jerusalem, Israel), GABA A R α3 (1:500; AGA-003, Alomone, Jerusalem, Israel), GABA A R α4 (1:200; AGA-008, Alomone, Jerusalem, Israel), GABA A R α5 (1:1000; AB9678, Sigma-Aldrich, St. Louis, MO, USA), GABA A R β2 (1:1000; ab8340, Abcam, Cambridge, UK), GABA A R β3 (1:1000; ab4046, Abcam, Cambridge, UK), GABA A R γ2 (1:200; AGA-005, Alomone, Jerusalem, Israel), and GABA A R δ (1:200; AGA-014, Alomone, Jerusalem, Israel) with β-actin (1:1000; ab8226, Abcam, Cambridge UK) used for normalization.

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Altered GABA A Receptor Expression in the Primary Somatosensory Cortex of a Mouse Model of Genetic Absence Epilepsy

doi: 10.3390/ijms232415685

Figure Lengend Snippet: WB analysis for the principal tonic GABA A R α4, α5, and δ subunits. Analysis compares the primary SoCx of control (NE) littermates and the epileptic (E) stargazers. ( A – C ) Representative blots display tissue expression of GABA A R α4, α5, and δ subunits in the primary SoCx with β-actin as the loading control. Bar graphs represent the relative expression levels of GABA A R α4, α5 and δ subunits, respectively. Bar graphs reveal a lack of statistically significant change in whole-tissue primary SoCx expression levels for GABA A R α4 (NE: 1.000 ± 0.063 [ n = 18], E: 1.117 ± 0.095 [ n = 15]; p = 0.325), α5 (NE: 1.000 ± 0.048 [ n = 23], E: 0.937 ± 0.083 [ n = 13]; p = 0.672), and δ (NE: 1.000 ± 0.041 [ n = 27], E: 0.899 ± 0.069 [ n = 23]; p = 0.215). The significance threshold was set at 0.05. ‘ns’ indicates no significant change found from analyses.

Article Snippet: Membranes were probed using GABA A R α1 (1:500; AGA-001, Alomone, Jerusalem, Israel), GABA A R α3 (1:500; AGA-003, Alomone, Jerusalem, Israel), GABA A R α4 (1:200; AGA-008, Alomone, Jerusalem, Israel), GABA A R α5 (1:1000; AB9678, Sigma-Aldrich, St. Louis, MO, USA), GABA A R β2 (1:1000; ab8340, Abcam, Cambridge, UK), GABA A R β3 (1:1000; ab4046, Abcam, Cambridge, UK), GABA A R γ2 (1:200; AGA-005, Alomone, Jerusalem, Israel), and GABA A R δ (1:200; AGA-014, Alomone, Jerusalem, Israel) with β-actin (1:1000; ab8226, Abcam, Cambridge UK) used for normalization.

Techniques: Control, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Altered GABA A Receptor Expression in the Primary Somatosensory Cortex of a Mouse Model of Genetic Absence Epilepsy

doi: 10.3390/ijms232415685

Figure Lengend Snippet: WB analyses of biochemically isolated fractions from primary SoCx for GABA A R α4, α5, and δ subunits. Subcellular fractions were isolated from the control (NE) littermates and epileptic epileptic (E) stargazers. ( A – D ) Biochemical fractionation analysis revealed no significant change in GABA A R α4 in all subcellular components from the primary SoCx of stargazers compared to their NE littermates: total lysate (NE: 1.000 ± 0.063 [ n = 9], E: 1.353 ± 0.227 [ n = 9]; p = 0.257), cytosol (NE: 1.000 ± 0.088 [ n = 14], E: 1.308 ± 0.223 [ n = 10]; p = 0.172), extra-synaptic (NE: 1.000 ± 0.051 [ n = 14], E: 0.789 ± 0.183 [ n = 9]; p = 0.516), and synaptic (NE: 1.000 ± 0.047 [ n = 14], E: 0.955 ± 0.122 [ n = 9]; p = 0.369). ( E – H ) GABA A R α5 analysis revealed no significant change in all subcellular components from the primary SoCx of stargazers compared to their control littermates: total lysate (NE: 1.000 ± 0.094 [ n = 10], E: 0.919 ± 0.102 [ n = 10]; p = 0.566), cytosol (NE: 1.000 ± 0.113 [ n = 14], E: 1.295 ± 0.362 [ n = 8]; p = 0.920), extra-synaptic (NE: 1.000 ± 0.068 [ n = 13], E: 1.324 ± 0.214 [ n = 9]; p = 0.431) and, synaptic (NE: 1.000 ± 0.075 [ n = 13], E: 1.190 ± 0.195 [ n = 9]; p = 0.744). ( I – L ) No statistically significant change was also seen for GABA A R δ subunit in all subcellular components from the primary SoCx of stargazers compared to their control littermates: total lysate (NE: 1.000 ± 0.071 [ n = 15], E: 1.088 ± 0.201 [ n = 9]; p = 0.815), cytosol (NE: 1.000 ± 0.083 [ n = 15], E: 0.831 ± 0.138 [ n = 9]; p = 0.379), extra-synaptic (NE: 1.000 ± 0.078 [ n = 15], E: 1.419 ± 0.241 [ n = 10]; p = 0.191), and synaptic (NE: 1.000 ± 0.053 [ n = 15], E: 1.092 ± 0.094 [ n = 9]; p = 0.263). The significance threshold was set at 0.05. ‘ns’ indicates no significant change found from analyses.

Article Snippet: Membranes were probed using GABA A R α1 (1:500; AGA-001, Alomone, Jerusalem, Israel), GABA A R α3 (1:500; AGA-003, Alomone, Jerusalem, Israel), GABA A R α4 (1:200; AGA-008, Alomone, Jerusalem, Israel), GABA A R α5 (1:1000; AB9678, Sigma-Aldrich, St. Louis, MO, USA), GABA A R β2 (1:1000; ab8340, Abcam, Cambridge, UK), GABA A R β3 (1:1000; ab4046, Abcam, Cambridge, UK), GABA A R γ2 (1:200; AGA-005, Alomone, Jerusalem, Israel), and GABA A R δ (1:200; AGA-014, Alomone, Jerusalem, Israel) with β-actin (1:1000; ab8226, Abcam, Cambridge UK) used for normalization.

Techniques: Isolation, Control, Fractionation